Western Orchids / Laboratories

Tissue Culture Medium

 

Our medium has been under continuous development for over 38 years. We use our own media exclusively and we know they work and we can make recommendations based on our own practical experience.

Our medium is complete – it comes as a small sachet of dry powder.
It includes macro and micro electrolytes and organics, agar and sugar.
It has an intrinsic buffering capacity and a full range of essential trace elements. If the medium is intended for explant start [mericloning] it also includes essential Plant Growth Regulators. In all cases you only need to add water; banana pulp and / or coconut water when needed.


Western Orchids Tissue Culture Medium

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Packs of tissue culture medium

Food Jar

Freshly set agar
   
 
   

 

 

Media Prices

(subject to change without notice) Does NOT include GST

1 – 14 litres $7.50 /litre
15 – 50 litres $7.00 /litre
51 – 100 litres $6.50 /litre
Over 100 litres $6.00 /litre
Over 100 litres in bulk packs $5.50 /litre

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Postage is included in price when 5 or more litres are ordered – sent by Express Post. (Medium can also be supplied as bulk packs of 2litres, 5litres, 10litres, 100litres, etc)

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Storage

Store packs of Dry Powder Medium in a freezer until needed. Shelf life at least 10 years frozen.

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Preparation

For a 1 litre lot, to a blender add 200 to 250 mLs of just boiled water, followed immediately by the packet of tissue culture medium
- Blend for about 20 to 30 seconds until it thickens
- Add more just boiled water to about 500mL and blend momentarily
- Add banana (and / or coconut milk where indicated)
- Blend again until homogenised [ up to a minute at most ]
- Add more water to 1 litre ( or to 1,100 mL for a softer better to use mix )
- Blend until just mixed
- Pour into suitable flasks. Seal with suitable closures and sterilise.

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Hormone Keiki paste

Price: $8 (plus gst where applicable) per 4ml tube

Read more about our Hormone Keiki Paste >>

 

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We welcome all enquiries regarding use of our medium and its use in
aseptic tissue culture.

Overseas orders very welcome.

LIST OF TISSUE CULTURE MEDIUM >>

INFORMATION ON SOWING & REPLATE MEDIUM >>

PREPARATION & STERILISATION OF MEDIUM >>

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Western Orchids / Laboratories
PO Box 276 , Blackwood, South Australia , 5051
Phone / Fax: 08 8270 4599
Email:

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