Western Orchids Laboratory

Tissue Culture Medium Formulations


Our medium has been under continuous development for over 38 years. We use our own media exclusively and we know they work and we can make recommendations based on our own practical experience.

Our medium is complete – it comes as a small sachet of dry powder.
It includes macro and micro electrolytes and organics, agar and sugar.
It has an intrinsic buffering capacity and a full range of essential trace elements. If the medium is intended for explant start [mericloning] it also includes essential Plant Growth Regulators. In all cases you only need to add water; banana pulp and / or coconut water when needed.

Media Prices

(subject to change without notice) Does NOT include GST
1 – 14 litres $7.50
15 – 50 litres $7.00
51 - 100 litres $6.50
Over 100 litres [packed in 5 litre packs or greater] $6.00
Over 100 litres in bulk packs $5.50

Postage is included in price when 5 or more litres are ordered – sent by Express Post. (Medium can also be supplied as bulk packs of 2litres, 5litres, 10litres, 100litres, etc)

Click here to ORDER

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Storage

Store packs of Dry Powder Medium in a freezer until needed. Shelf life at least 10 years frozen.

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We use and market the following media formulations that are giving ourselves & customers extremely satisfactory results. No tissue culture operator can possibly have sown or replated every genus or species that enthusiasts may intend to work with. Within the one species or genus it is possible to find that some prefer different conditions from what has come to be expected.

Many orchids will grow happily on a vast range of media so it is not possible to make absolute recommendationson what medium will perfectly suit which orchids. Moreover, it is better to use as few media as possible to avoid confusion and wastage so the following recommendations will be seen to have some overlap but this is what we have found in our years of experience.

Our media are complete, needing the addition only of media quality water and may have banana pulp & / or coconut water &/or pineapple juice added. [ see below & refer to the ‘Media Information’ sheet ] we also work on the premise that the best customers are those who get good results and we are happy to discuss any media-related problems to help you get the best possible results with your tissue culture activities.
[ Note" All but ‘fluid formulations’ have agar already included in them ]

 

MEDIA FORMULATIONS

W1 – CYMBIDIUMS, DENDROBIUM AND CATTLEYA ALLIANCE
This medium, with the addition of 60grams of banana pulp per litre is excellent both for seed sowing, respreading and final replate of Cymbidiums producing large, well proportioned plants with ideal root growth. This product is ideal for the Cymbidium, Dendrobium and Cattleya alliance growers who want the simplicity of one medium for all purposes. Explant start & cloning of Cymbidiums can be achieved also on WI but W4S#Z is absolute magic for this purpose.

W2 - GENERAL PURPOSE SOW AND REPLATE FORMULA
This product, with different additives can be used to sow or replate a very wide range of orchid genera. W2 with banana pulp added @ 60grams/liter is especially suited to germinate & replate : Dendrobiums, Cattleya alliance & vigorous Oncidium / Miltonia / Odontoglossum alliance. It may also be used to germinate and grow Cymbidium , Sarcochilus, Disa & Masdevallia. ALWAYS USE banana pulp @ 60g/L for REPLATE WORK. GERMINATION MAY BE ENHANCED by using W2 with COCONUT WATER at 20 – 30mL /Litre from fresh, unspoiled coconuts or 30mL of 'Golden Circle' tinned, unsweetened, unpreserved PINEAPPLE JUICE per litre together with, in each case about 30grams of banana pulp / Litre also. [ Use pineapple juice only if you have access to a pH meter as it is very acidic & pH must be properly readjusted after its addition ]

W2.5 - GENERAL PURPOSE SOW & REPLATE FORMULA
This product is satisfactory for the same genera as W2 above but is good also for GERMINATION AND REPLATE of Phalaenopsis, Sarcochilus, Vanda alliance, Disa, Masdevallia, Lycaste, Maxillaria, Terrestrial Orchids and is probably equally effective as W2 for Dendrobium. We recommend W2.5 for use with crosses that have a high tendency to clone themselves after germination. Use banana pulp / coconut water / pineapple juice as described for W2 above.

W1.5 – Born from modifications to W1
Proving to be superior to W2.5 in some cases where W2.5 would normally be used. Some customers have better results with W1.5 whilst others prefer W2.5. We think it tends to depend on the particular line of orchids one is using. We have had good results with Paphiopedilum replates in particular and with dendrobiums or oncidium alliance plants that tend to keep multiplying after final replate.

W3 - ALL ROUNDER
This is the true ALL-ROUND SOWING/GERMINATING medium and has given excellent results with almost every genus tested to date it is especially good for Zygopetalum and Lycaste alliance. If in doubt, use W3 with banana pulp at 60grams / litre. If seeds fail to germinate or only a few on this formula either the seed was not live or treat as for Paphiopedilum [ below ] or try W3 + Coconut Water @ 20mL / Litre + banana pulp @ 40 grams / litre
W3 with COCONUT WATER @ 20mL / Litre or PINEAPPLE JUICE @ 30mL / Litre & pH of 5.5 to 5.8 GERMINATED ALL GENERA TESTED INCLUDING PAPHIOPEDILUM & PHRAGMIPEDIUM QUICKLY & EXTENSIVELY. W3 with banana pulp at 60grams/Litre IS EXCELLENT FOR REPLATE OF PAPHIOPEDILUM & PHRAGMIPEDIUM. Seedlings germinated on W3 with coconut water &/or pineapple juice must be respread on to W3 + banana pulp quite soon after germinating. W3 with banana pulp at 60grams / Litre can be used if required as a general purpose REPLATE medium for all genera especially Phalaenopsis, terrestrials & Vanda alliance but may be slower than W1, W2 or W2.5 or W1.5 for other genera.

WCATT New formula!
Specifically designed to promote good and rapid growth in respreads and final replates of seedlings and clones in Cattleya and cattleya alliance and to minimise autocloning. Results in our laboratory and that of customers involved has been very good. Also really great for replates of Aust dendrobium species & hybrids, dockrillia and cymbidium. Our laboratory is progressively using this formula in place of our other previously commonly used formulations. Not for sowing!! Test it also on Sarcochilus, sophronitis, phalaenopsis and Disa.

W4#Z - ALL PURPOSE EXPLANT START & CLONING FORMULA
Particularly suited to explant start & OK for multiplication of CYMBIDIUM, ONCIDIUM/MILTONIA ALLIANCE, and seems to be the most effective yet seen on CATTLEYA ALLIANCE ALSO. Solid format will start Phalaenopsis flower stems very well. Available in 2 forms: W4S#Z SOLID CLONING AND W4F#Z FLUID FORMAT.

W4M - CYMULTIPLY
Mutiplication formula to rapidly multiply tissue of cymbidium, CATTLEYA and Cattleya allaince, & oncidium/miltonia alliance - solid or liquid formats available - W4MS & W4MF. Solid generally best for general use but commercial labs may prefer fluid format.

W6FS ## - Phalaenopsis FLOWER-STEM propagation medium.
Specifically formulated to initiate vegetative growths on flower stem nodes in culture. $8.00 per litre.

W6L ## Used to produce protocorms from tissue produced on W6FS.

NOTE: Media with ## ARE ACCOMPANIED BY PROCESS NOTES TO OUTLINE SPECIFIC MEDIA & CLONING PROCESS STEPS INVOLVED.

Our media are formulated to set perfectly for handling & replating with the set strength that has been arrived at after 38 years of experience. My media set slightly on the soft side to promote ready uptake of water & nutrients by the orchids. It is imperative that sterilization temperatures DO NOT EXCEED 123 deg Centigrade and that once the Autoclave or Pressure cooker cycle is finished and it has reached room pressure, the flasks of medium must be removed and placed in a cool place to cool & set. Prolonged exposure to high temperatures reduces media potency & weakens the agar. If a harder set is required, please specify when ordering & increased agar will be added without extra cost.

WE USE THE MEDIUM IN OUR OWN LABORATORY AND CONTINUOUSLY SEEK TO MAXIMISE ITS PERFORMANCE. IF YOU LIKE OUR MEDIA, PLEASE TELL YOUR FRIENDS. IF YOU HAVE A PROBLEM, PLEASE CONTACT US FOR HELP .

Freight charged at cost to overseas customers.

Media orders of 5 or more litres sent Express post in Australia.
All orders attended to promptly.

 

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Western Orchids / Laboratories
PO Box 276 , Blackwood, South Australia , 5051
Phone / Fax: 08 8270 4599
Email:

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